gene exp peg10 hs01122880 m1 (Thermo Fisher)

Thermo Fisher gene exp peg10 hs01122880 m1

<t>PEG10</t> mRNA expression in placental lysates from patients with early-onset preeclampsia. PEG10 mRNA expression was reduced in placentas from 79 women with early-onset preeclampsia (pink line) compared to 18 gestation-matched preterm controls (grey) ( A ). Further analysis of the early-onset preeclampsia cohort (blue circle) and gestation-matched pre-term controls (grey diamond) showed no significant association between gestation ( B ) or birth weight ( C ). Data expressed as a median with interquartile range. Simple linear regression was used to present a line of best fit (solid line) and 95% confidence interval (dashed line). Individual symbols represent individual patients. P * < 0.05

Gene Exp Peg10 Hs01122880 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peg10+gene/Gene+Exp%2E+PEG10%2C+Hs01122880_m1/pmc10355026-107-32-5
Average 85 stars, based on 1 article reviews

gene exp peg10 hs01122880 m1 - by Bioz Stars, 2026-10

85/100 stars

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human peg10 gene orf cdna clone expression plasmid, c-gfpspark tag (Sino Biological)

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Full length Clone DNA of Human paternally expressed 10 with C terminal GFPSpark tag.

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gene exp peg10 hs00248288 s1 (Thermo Fisher)

Thermo Fisher gene exp peg10 hs00248288 s1

Gene Exp Peg10 Hs00248288 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peg10+gene/Gene+Exp%2E+PEG10%2C+Hs00248288_s1/pmc06614557-910-71-31
Average 85 stars, based on 1 article reviews

gene exp peg10 hs00248288 s1 - by Bioz Stars, 2026-10

85/100 stars

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gene exp peg10 mm01167724 m1 (Thermo Fisher)

Thermo Fisher gene exp peg10 mm01167724 m1

Validation and proteasome dependence of top hits in HEK 293 cells . A , western blot demonstrating similar changes in TRIM32, <t>PEG10,</t> and RTL8 protein levels in HEK 293 cells lacking UBQLN1, 2, and 4 (labeled TKO). B , WT or triple knockout (TKO) HEK 293 cells were treated with bortezomib plus epoxomicin (50 nM each) or chloroquine (50 μM), for 0, 3, 6, or 12 h to inhibit proteasomal or lysosomal degradation, respectively. Inhibition of degradation was confirmed with either poly-ubiquitin (Ub) accumulation and free ubiquitin (Ub) depletion or LC3-I/II conversion, respectively. Western blots were probed for top UBQLN2 hits. C–E , WT or TKO HEK 293 cells were transfected with constructs bearing C-terminal fusions of ( C ) PEG10-RF1/2, ( D ) RTL8C, and ( E ) TRIM32 with Dendra2 fluorophore with an IRES-CFP transfection control. After 48 h, cells were exposed to 60 s of intense blue light to induce photoconversion of Dendra2 and samples were taken at the indicated timepoints for quantitation of the RFP/CFP ratio via flow cytometry. Lines of best fit to biphasic decay were plotted in Prism for each time course. n = 4 independent experiments for each construct. RTL8 overexp., overexposure of RTL8 blot.

Gene Exp Peg10 Mm01167724 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peg10+gene/Gene+Exp%2E+Peg10%2C+Mm01167724_m1/pmc07873701-419-17--1
Average 90 stars, based on 1 article reviews

gene exp peg10 mm01167724 m1 - by Bioz Stars, 2026-10

90/100 stars

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peg10 rf1/2 antibody (iCell Gene Therapeutics)

iCell Gene Therapeutics peg10 rf1/2 antibody

Peg10 Rf1/2 Antibody, supplied by iCell Gene Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peg10+gene/peg10+rf1+2+antibody/pmc08385294__mmc1-51-1-15
Average 90 stars, based on 1 article reviews

peg10 rf1/2 antibody - by Bioz Stars, 2026-10

90/100 stars

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anti-peg10: mouse paternally expressed gene-10 antibody (Cell Applications Inc)

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BACKGROUND The Paternally Expressed Gene-10 (PEG10) gene was identified based on its location in an imprinted domain on human chromosome 7q21 and characterized as paternally expressed/maternally silenced. PEG10 is also imprinted in mice. The major

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human peg10 gene orf cdna clone in cloning vector (Sino Biological)

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Full length Clone DNA of Human paternally expressed 10.

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human peg10 gene orf cdna clone expression plasmid, c-myc tag (Sino Biological)

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Full length Clone DNA of Human paternally expressed 10 with C terminal Myc tag.

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human peg10 gene orf cdna clone expression plasmid, n-his tag (Sino Biological)

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Full length Clone DNA of Human paternally expressed 10 with N terminal His tag.

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human peg10 gene orf cdna clone expression plasmid, c-ha tag (Sino Biological)

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Full length Clone DNA of Human paternally expressed 10 with C terminal HA tag.

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human peg10 gene orf cdna clone expression plasmid, n-myc tag (Sino Biological)

N/A

Full length Clone DNA of Human paternally expressed 10 with N terminal Myc tag.

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Image Search Results

PEG10 mRNA expression in placental lysates from patients with early-onset preeclampsia. PEG10 mRNA expression was reduced in placentas from 79 women with early-onset preeclampsia (pink line) compared to 18 gestation-matched preterm controls (grey) ( A ). Further analysis of the early-onset preeclampsia cohort (blue circle) and gestation-matched pre-term controls (grey diamond) showed no significant association between gestation ( B ) or birth weight ( C ). Data expressed as a median with interquartile range. Simple linear regression was used to present a line of best fit (solid line) and 95% confidence interval (dashed line). Individual symbols represent individual patients. P * < 0.05

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia

doi: 10.1186/s12958-023-01116-3

Figure Lengend Snippet: PEG10 mRNA expression in placental lysates from patients with early-onset preeclampsia. PEG10 mRNA expression was reduced in placentas from 79 women with early-onset preeclampsia (pink line) compared to 18 gestation-matched preterm controls (grey) ( A ). Further analysis of the early-onset preeclampsia cohort (blue circle) and gestation-matched pre-term controls (grey diamond) showed no significant association between gestation ( B ) or birth weight ( C ). Data expressed as a median with interquartile range. Simple linear regression was used to present a line of best fit (solid line) and 95% confidence interval (dashed line). Individual symbols represent individual patients. P * < 0.05

Article Snippet: Taqman Fast Advanced Master Mix (ThermoFisher Scientific, Waltham, USA) and specific fluorescein amidite (FAM)-labelled Taqman gene expression assays (Life Technologies, Carlsbad, USA) were used to measure mRNA expression of PEG10 (Assay ID: Hs01122880_m1), CDH2 (n-cadherin 2, Assay ID: Hs00983056_m1), GATA3 (GATA binding protein 3, Assay ID: Hs00231122_m1), HLAG (human leukocyte antigen G, Assay ID: Hs03045108_m1), SDC1 (syndecan-1, Assay ID: Hs00896423_m1) and TEAD4 (TEA domain transcription factor 4, Assay ID: Hs01125032_m1).

Techniques: Expressing

$Localisation of PEG10 protein and mRNA within the placenta. Immunohistochemistry (IHC) staining of human placental villi shows PEG10 protein (brown) localised to cytotrophoblasts in both control and early-onset preeclampsia tissue ( A ). Semi-quantitative, blinded assessment of IHC staining indicated reduced PEG10 protein in early-onset preeclampsia placentas relative to controls ( C ). In situ hybridisation shows PEG10 (blue) and PECAM-1 (pink) mRNA expression. PEG10 expression localised to cytotrophoblasts in both control and early-onset preeclampsia tissues while PECAM-1 marked placental vasculature ( B ). No staining observed in respective isotype controls (minimised image). Figures were captured at 40 × magnification and are representative of 5 experimental repeats. Data expressed as mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\pm$$\end{document} ± SEM. Individual symbols represent individual scores and P * < 0.05$

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia

doi: 10.1186/s12958-023-01116-3

Figure Lengend Snippet: Localisation of PEG10 protein and mRNA within the placenta. Immunohistochemistry (IHC) staining of human placental villi shows PEG10 protein (brown) localised to cytotrophoblasts in both control and early-onset preeclampsia tissue ( A ). Semi-quantitative, blinded assessment of IHC staining indicated reduced PEG10 protein in early-onset preeclampsia placentas relative to controls ( C ). In situ hybridisation shows PEG10 (blue) and PECAM-1 (pink) mRNA expression. PEG10 expression localised to cytotrophoblasts in both control and early-onset preeclampsia tissues while PECAM-1 marked placental vasculature ( B ). No staining observed in respective isotype controls (minimised image). Figures were captured at 40 × magnification and are representative of 5 experimental repeats. Data expressed as mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\pm$$\end{document} ± SEM. Individual symbols represent individual scores and P * < 0.05

Techniques: Immunohistochemistry, Control, In Situ, Hybridization, Expressing, Staining

PEG10 gene expression reduces with cytotrophoblast differentiation. First-trimester human cytotrophoblast stem cells (hTSCs) were differentiated into syncytiotrophoblasts (ST) over 96 h ( A-D ) . Effective syncytialisation was confirmed by a decrease in trophoblast markers, TEAD4 (A) and CDH2 ( B ) and an increase in ST marker, SDC1 ( C ). PEG10 mRNA expression significantly decreased by 96 h in ST cells ( D ). hTSCs were differentiated into extravillous trophoblast (EVT) cells across 144 h as shown by an increase in EVT marker, HLAG ( E ). PEG10 mRNA expression was significantly decreased after 144 h of EVT differentiation ( F ). Data expressed as a median with interquartile range. Individual symbols represent n = 5 experimental repeats. P ** < 0.01, P *** < 0.001

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia

doi: 10.1186/s12958-023-01116-3

Figure Lengend Snippet: PEG10 gene expression reduces with cytotrophoblast differentiation. First-trimester human cytotrophoblast stem cells (hTSCs) were differentiated into syncytiotrophoblasts (ST) over 96 h ( A-D ) . Effective syncytialisation was confirmed by a decrease in trophoblast markers, TEAD4 (A) and CDH2 ( B ) and an increase in ST marker, SDC1 ( C ). PEG10 mRNA expression significantly decreased by 96 h in ST cells ( D ). hTSCs were differentiated into extravillous trophoblast (EVT) cells across 144 h as shown by an increase in EVT marker, HLAG ( E ). PEG10 mRNA expression was significantly decreased after 144 h of EVT differentiation ( F ). Data expressed as a median with interquartile range. Individual symbols represent n = 5 experimental repeats. P ** < 0.01, P *** < 0.001

Techniques: Gene Expression, Marker, Expressing

The effect of hypoxia and inflammatory cytokines on PEG10 expression in cytotrophoblast stem cells. First-trimester human cytotrophoblast stem cells (hTSCs) or term primary cytotrophoblast were exposed to ( A-B ). PEG10 mRNA expression was significantly reduced in hTSCs exposed to hypoxia (1% oxygen) relative to normoxia (8% oxygen) ( A ). A similar finding was observed when primary term trophoblast were exposed to hypoxia ( B ). Next, hTSCs were treated with increasing doses of TNFα (0, 0.1, 1 and 10 ng/mL) and showed a dose-dependent reduction in PEG10 expression when compared to vehicle-treated controls ( C ). No changes in PEG10 expression were observed in cells with increasing doses (0, 0.1, 1, 10 ng/mL) of IL-6 ( D ). Data is presented as a median with interquartile range. Individual symbols represent n = 5 experimental repeats and P ** < 0.01

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia

doi: 10.1186/s12958-023-01116-3

Figure Lengend Snippet: The effect of hypoxia and inflammatory cytokines on PEG10 expression in cytotrophoblast stem cells. First-trimester human cytotrophoblast stem cells (hTSCs) or term primary cytotrophoblast were exposed to ( A-B ). PEG10 mRNA expression was significantly reduced in hTSCs exposed to hypoxia (1% oxygen) relative to normoxia (8% oxygen) ( A ). A similar finding was observed when primary term trophoblast were exposed to hypoxia ( B ). Next, hTSCs were treated with increasing doses of TNFα (0, 0.1, 1 and 10 ng/mL) and showed a dose-dependent reduction in PEG10 expression when compared to vehicle-treated controls ( C ). No changes in PEG10 expression were observed in cells with increasing doses (0, 0.1, 1, 10 ng/mL) of IL-6 ( D ). Data is presented as a median with interquartile range. Individual symbols represent n = 5 experimental repeats and P ** < 0.01

Techniques: Expressing

Effect of silenced PEG10 on trophoblast differentiation. First-trimester human cytotrophoblast stem cells (hTSCs) were treated with PEG10 siRNA prior to differentiation into syncytiotrophoblasts over 96 h. No change in the expression of hTSC and syncytiotrophoblast markers, CDH2 ( A ) and SDC1 ( B ) were observed in PEG10 siRNA-treated cells, relative to negative controls. Next, PEG10-silenced hTSCs were differentiated into EVTs over 144 h. No change in hTSC or EVT markers, TEAD4 ( C ) and HLAG ( D ) were observed in PEG10 siRNA-treated cells, compared to controls. Data expressed as a median with interquartile range. Individual symbols represent n = 5 experimental repeats

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia

doi: 10.1186/s12958-023-01116-3

Figure Lengend Snippet: Effect of silenced PEG10 on trophoblast differentiation. First-trimester human cytotrophoblast stem cells (hTSCs) were treated with PEG10 siRNA prior to differentiation into syncytiotrophoblasts over 96 h. No change in the expression of hTSC and syncytiotrophoblast markers, CDH2 ( A ) and SDC1 ( B ) were observed in PEG10 siRNA-treated cells, relative to negative controls. Next, PEG10-silenced hTSCs were differentiated into EVTs over 144 h. No change in hTSC or EVT markers, TEAD4 ( C ) and HLAG ( D ) were observed in PEG10 siRNA-treated cells, compared to controls. Data expressed as a median with interquartile range. Individual symbols represent n = 5 experimental repeats

Techniques: Expressing

$Effect of silenced PEG10 on cytotrophoblast function. Following transfection with luciferase reporter plasmid containing four copies of the SMAD binding element, PEG10 -siRNA hTSCs had reduced basal levels of canonical TGF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$-\beta$$\end{document} - β signalling relative to negative siRNA controls ( A ). Real-time analysis of cell proliferation showed no effect of PEG10 siRNA (pink) relative to control siRNA treated cells (black) ( B ). Dotted line in panel B indicates time of siRNA treatment, dashed line indicates time of media change. Data expressed as median with interquartile range. P * < 0.05$

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia

doi: 10.1186/s12958-023-01116-3

Figure Lengend Snippet: Effect of silenced PEG10 on cytotrophoblast function. Following transfection with luciferase reporter plasmid containing four copies of the SMAD binding element, PEG10 -siRNA hTSCs had reduced basal levels of canonical TGF \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$-\beta$$\end{document} - β signalling relative to negative siRNA controls ( A ). Real-time analysis of cell proliferation showed no effect of PEG10 siRNA (pink) relative to control siRNA treated cells (black) ( B ). Dotted line in panel B indicates time of siRNA treatment, dashed line indicates time of media change. Data expressed as median with interquartile range. P * < 0.05

Techniques: Transfection, Luciferase, Plasmid Preparation, Binding Assay, Control

Validation and proteasome dependence of top hits in HEK 293 cells . A , western blot demonstrating similar changes in TRIM32, PEG10, and RTL8 protein levels in HEK 293 cells lacking UBQLN1, 2, and 4 (labeled TKO). B , WT or triple knockout (TKO) HEK 293 cells were treated with bortezomib plus epoxomicin (50 nM each) or chloroquine (50 μM), for 0, 3, 6, or 12 h to inhibit proteasomal or lysosomal degradation, respectively. Inhibition of degradation was confirmed with either poly-ubiquitin (Ub) accumulation and free ubiquitin (Ub) depletion or LC3-I/II conversion, respectively. Western blots were probed for top UBQLN2 hits. C–E , WT or TKO HEK 293 cells were transfected with constructs bearing C-terminal fusions of ( C ) PEG10-RF1/2, ( D ) RTL8C, and ( E ) TRIM32 with Dendra2 fluorophore with an IRES-CFP transfection control. After 48 h, cells were exposed to 60 s of intense blue light to induce photoconversion of Dendra2 and samples were taken at the indicated timepoints for quantitation of the RFP/CFP ratio via flow cytometry. Lines of best fit to biphasic decay were plotted in Prism for each time course. n = 4 independent experiments for each construct. RTL8 overexp., overexposure of RTL8 blot.

Journal: The Journal of Biological Chemistry

Article Title: Global proteomics of Ubqln 2-based murine models of ALS

doi: 10.1074/jbc.RA120.015960

Figure Lengend Snippet: Validation and proteasome dependence of top hits in HEK 293 cells . A , western blot demonstrating similar changes in TRIM32, PEG10, and RTL8 protein levels in HEK 293 cells lacking UBQLN1, 2, and 4 (labeled TKO). B , WT or triple knockout (TKO) HEK 293 cells were treated with bortezomib plus epoxomicin (50 nM each) or chloroquine (50 μM), for 0, 3, 6, or 12 h to inhibit proteasomal or lysosomal degradation, respectively. Inhibition of degradation was confirmed with either poly-ubiquitin (Ub) accumulation and free ubiquitin (Ub) depletion or LC3-I/II conversion, respectively. Western blots were probed for top UBQLN2 hits. C–E , WT or TKO HEK 293 cells were transfected with constructs bearing C-terminal fusions of ( C ) PEG10-RF1/2, ( D ) RTL8C, and ( E ) TRIM32 with Dendra2 fluorophore with an IRES-CFP transfection control. After 48 h, cells were exposed to 60 s of intense blue light to induce photoconversion of Dendra2 and samples were taken at the indicated timepoints for quantitation of the RFP/CFP ratio via flow cytometry. Lines of best fit to biphasic decay were plotted in Prism for each time course. n = 4 independent experiments for each construct. RTL8 overexp., overexposure of RTL8 blot.

Article Snippet: TaqMan primer/probe sets for mouse tissues were as follows: Gapdh (VIC): Mm99999915_g1, Trim32 (MGB): Mm00551733_m1, Peg10 (MGB): Mm01167724_m1, Cxx1b (MGB): Mm03032892_gH.

Techniques: Western Blot, Labeling, Triple Knockout, Inhibition, Transfection, Construct, Quantitation Assay, Flow Cytometry

peg10 gene Search Results

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